Composite
Part:BBa_K2443024:Design
Designed by: Lethbridge iGEM 2017 Group: iGEM17_Lethbridge (2017-10-18)
IF2 optimized for expression in E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2345
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1700
Illegal AgeI site found at 311 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1031
Illegal SapI site found at 966
Design Notes
Codon optimized for use in Escherichia coli. All regulatory elements are also optimal for use within Escherichia coli
Source
see basic parts